Ultraviolet-Visible (UV-Vis) Spectroscopy UV-Visible spectroscopy is a branch of analytical chemistry that employs the absorption of light within the range...
Ultraviolet-Visible (UV-Vis) Spectroscopy
UV-Visible spectroscopy is a branch of analytical chemistry that employs the absorption of light within the range of wavelengths between 200 and 800 nm to analyze and identify compounds. The absorption of light by molecules occurs due to the electronic transitions between different energy levels within the molecule. By measuring the amount and wavelength of the absorbed light, it is possible to obtain information about the molecular structure, composition, and dynamics of the sample.
Infrared (IR) Spectroscopy
IR spectroscopy is another spectroscopic technique that utilizes the absorption and emission of infrared radiation by molecules to determine their molecular structure and properties. The infrared region of the spectrum covers a range of frequencies from approximately 4000 to 400 cm−1. Absorption in the IR region is attributed to the vibrational motion of molecules, and the frequency of absorption corresponds to the energy of the photon absorbed or emitted. By analyzing the IR spectrum, it is possible to identify the functional groups present in the molecule, determine molecular weight and molecular geometry, and gain insights into the electronic structure and bonding patterns